Bioseparations Principles and Techniques by B. Sivasankar: A Comprehensive and Practical Guide for Students and Professionals
Bioseparations, also known as downstream processing, is a crucial step in the production of biotechnology products, such as vaccines, enzymes, hormones, antibodies, and drugs. Bioseparations involves the separation, purification, and characterization of biomolecules from complex mixtures, such as cell cultures, fermentation broths, blood, and tissue extracts.
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However, bioseparations is not only a scientific challenge, but also an economic one. The cost of bioseparations can account for up to 80% of the total production cost of a biotechnology product. Therefore, it is essential to optimize the bioseparations process to achieve high yield, purity, and quality of the desired product, while minimizing the waste generation and environmental impact.
One of the most comprehensive and practical books on bioseparations is Bioseparations Principles and Techniques by B. Sivasankar. This book covers the theoretical principles and techniques involved in bioseparations, such as filtration, centrifugation, adsorption, extraction, membrane separation, chromatography, electrophoresis, and related methods. The book also highlights the important applications of these techniques in various fields of biotechnology.
The book is divided into 15 chapters, each covering a specific topic in bioseparations. The first few chapters introduce the basic concepts of bioseparations, such as mass transfer, phase equilibria, diffusion, and transport phenomena. The next chapters discuss the various unit operations of bioseparations, such as filtration, centrifugation, adsorption, extraction, and membrane separation. The following chapters focus on the most widely used technique of bioseparations: chromatography. The book covers different types of chromatography, such as gel filtration, affinity and pseudoaffinity chromatography, ion-exchange chromatography, hydrophobic interaction chromatography, reversed-phase chromatography, and high-performance liquid chromatography. The book also explains the principles and applications of electrophoresis and related methods, such as gel electrophoresis, capillary electrophoresis, isoelectric focusing, and immunoelectrophoresis. The last chapter summarizes the recent advances and future trends in bioseparations.
The book is written in a clear and concise manner, with numerous examples, illustrations, tables, and diagrams to aid the understanding of the concepts and techniques. The book also provides end-of-chapter questions and problems to test the knowledge and skills of the readers. The book is suitable for undergraduate and postgraduate students of science and engineering who are interested in learning about bioseparations. The book is also a valuable reference for professionals and researchers who are involved in the development and optimization of bioseparations processes.
Bioseparations Principles and Techniques by B. Sivasankar is available as an eBook from PHI Learning Pvt. Ltd., which can be downloaded for free for 15 days from their website[^1^]. The book can also be purchased as a print book from Prentice-Hall of India[^2^] or other online platforms. 29c81ba772